Laboratoire
Equipe d’Accueil : B cells and Skin Biology
Intitulé de l’Unité : Institut de Recherche Saint Louis – INSERM U976
Nom du Responsable de l’Unité : Jean-Christophe BORIES
Nom du Responsable de l’Équipe : Dorian OBINO
Adresse : 1 Avenue Claude Vellefaux, 75010 Paris
Responsable de l’encadrement :
Tél : 06 26 76 28 90 E-mail : dorian.obino@inserm.fr
Résumé du stage :
The Obino lab at Institut de Recherche Saint Louis (IRSL) in Paris in seeking for a highly motivated M2 student to study the local contribution of skin-associated B cells in the regulation of cutaneous inflammation and fibrosis. The internship could open on a PhD position.
Join our endeavor to understand how B cells residing in the skin dialog with neighboring stromal and immune cells to regulate the local inflammatory balance and how this is dysregulated during inflammatory skin disorders. To achieve this, we combine the use of mouse models of Systemic Scleroderma (SSc), B-cell depleting strategies, multiparameter conventional and spectral flow cytometry, single-cell sequencing approaches, and in vitro co-cultures of mouse and human primary cells. Key results will be confirmed on human skin biopsies obtained from SSc patients, thanks to our tight on-site collaboration with clinicians.
The project, to be started early 2025, will be carried out in the young and dynamic ATIP-Avenir-funded team (« B cells and Skin Biology ») led by Dorian Obino at IRSL in the center of Paris (10ème arrondissement). The candidate will benefit from the excellent scientific and collaborative enrivonment of the research institute and the Hôpital Saint-Louis, including state-of-the-art technological platforms (flow cytometry, imaging, genomics, and pre-clinical mouse models).
We are looking for a candidate with a solid academic training in immunology and/or tissue physiopathology, and a strong
commitment to working autonomously and with live animals. Prior experience with mouse work would be appreciated but is not mandatory (on-site training will be provided). Collaborative and team spirit, as well as confident oral and written communication skills in English are expected.
To apply, please send your CV, a motivation letter and the contact details of 1 or 2 references (in one single PDF document)
to Dorian Obino (dorian.obino@inserm.fr). For more information, informal inquires can also be sent to the same e-mail address.
Dernières Publications en lien avec le projet :
1) Meizlish ML, Franklin RA, Zhou X, Medzhitov R. Tissue Homeostasis and Inflammation. Annual review of immunology. 2021;39:557-81.
2) Richmond JM, Harris JE. Immunology and skin in health and disease. Cold Spring Harb Perspect Med. 2014;4(12):a015339.
3) Thoreau B, Chaigne B, Mouthon L. Role of B-Cell in the Pathogenesis of Systemic Sclerosis. Front Immunol. 2022;13:933468.
4) Liossis SC, Staveri C. The Role of B Cells in Scleroderma Lung Disease Pathogenesis. Front Med (Lausanne). 2022;9:936182.
5) Debes GF, McGettigan SE. Skin-Associated B Cells in Health and Inflammation. J Immunol. 2019;202(6):1659- 66.
6) Suchanek O, Ferdinand JR, Tuong ZK, Wijeyesinghe S, Chandra A, Clauder AK, et al. Tissue-resident B cells orchestrate macrophage polarisation and function. Nat Commun. 2023;14(1):7081.
7) Lerman I, Mitchell DC, Richardson CT. Human cutaneous B cells: what do we really know? Ann Transl Med. 2021;9(5):440.
8) Geherin SA, Gomez D, Glabman RA, Ruthel G, Hamann A, Debes GF. IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require alpha4beta1 Integrin To Migrate between the Peritoneum and Inflamed Skin. J Immunol. 2016;196(6):2514-25.
Ce projet s’inscrit dans la perspective d’une thèse via une Bourse de l’Ecole doctorale HOB (Hématologie – Oncogenèse – Biothérapies – Université Paris Cité) ou d’Associations